Sindbis Virus Glycoprotein E1 is divided into two discrete domains at amino acid 129 by disulfide bridge connections.
The
E1 membrane glycoprotein of Sindbis virus contains structural and functional
domains, which are conformationally dependent upon the presence of
intramolecular disulfide bridges (1, 5). We have examined the disulfide bonds in
E1, and have determined that the E1 membrane glycoprotein contains two separate
sets of interconnecting disulfide linkages, which divide the protein into two
domains at amino acid 129. These separate sets of disulfides may stabilize and
define the structural and functional regions of the E1 protein.