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Mailing Address:
Dr. A. Clay Clark
Associate Professor
128 Polk Hall, CB 7622
Department of Molecular
& Structural Biochemistry
NC State University
Raleigh, NC 27695-7622
Send Email: Clay Clark
Lab: Rooms 339 & 341 Polk Hall
Office: Room 339A Polk Hall
919-515-5805 (office)
919-515-5806 (lab)
919-515-2047 (fax)
Caspase-3 Expression Vectors
Click on the link for the sequence of each plasmid
All genes are in plasmid pET21b unless otherwise specified
Plasmid |
Comments |
Reference |
| pHC332 | Wild-type procaspase-3 |
Pop et al. (2001) Biochemistry 40, 14224. |
| pHC33201 | Procaspase-3(C163S) | Pop et al. (2001) Biochemistry 40, 14224. |
| pHC33202 | Procaspase-3(C163S,V266E) | Pop et al. (2003) Biochemistry 42, 12311. |
| pHC33203 | Procaspase-3(V266H) | Pop et al. (2003) Biochemistry 42, 12311. |
| pHC33204 | Procaspase-3(V266E) | Pop et al. (2003) Biochemistry 42, 12311. |
| pHC33205 | Procaspase-3(C163S,V266H) | Pop et al. (2003) Biochemistry 42, 12311. |
| pHC33206 | Procaspase-3(C163S) without His-tag | No Reference |
| pHC33207 | Procaspase-3(D175A) | Bose et al. (2003) Biochemistry 42, 12298. |
| pHC33208 | Procaspase-3(D28A,D175A) | Bose et al. (2003) Biochemistry 42, 12298. |
| pHC33209 | Procaspase-3(D9A,D28A,D175A) | Bose et al. (2003) Biochemistry 42, 12298. |
| pHC33210 | Procaspase-3 (D9A,D28A,D175A,V266E) | Pop et al. (2003) Biochemistry 42, 12311. |
| pHC33211 | Procaspase-3 (D9A,D28A,D175A,V266H) | Pop et al. (2003) Biochemistry 42, 12311. |
| pHC33214 | Procaspase-3(E246A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33215 | Procaspase-3(C163S,E246A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33216 | Procaspase-3 (D9A,D28A,D175A,E246A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33217 | Procaspase-3(E248A) | No Reference |
| pHC33218 | Procaspase-3(C163S,E248A) | No Reference |
| pHC33219 | Procaspase-3 (D9A,D28A,D175A,E248A) | No Reference |
| pHC33231 | Procaspase-3(E167A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33232 | Procaspase-3(D169A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33233 | Procaspase-3(E173A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33238 | Procaspase-3(K242A) | Feeney et al. (2004) Biochemical Journal 384, 515. |
| pHC33239 | Procaspase-3(C163S,K242A) | Feeney et al. (2004) Biochemical Journal 384, 515. |
| pHC33240 | Procaspase-3 (D9A,D28A,D175A,K242A) | Feeney et al. (2004) Biochemical Journal 384, 515. |
| pHC33241 | Procaspase-3 (D9A,D28A,D175A,E167A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33242 | Procaspase-3(D9A) | Feeney et al. (2005) JBC 280, 39772. |
| pHC33243 | Procaspase-3(D9A,C163S) | Feeney et al. (2005) JBC 280, 39772. |
| pHC33246 | Procaspase-3 (D9A,D28A,D175A,E173A) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33250 | Procaspase-3(Y203F) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33251 | Procaspase-3(C163S,Y203F) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33252 | Procaspase-3 (D9A,D28A,D175A,Y203F) | Feeney et al. (2006) Biochemistry 45, 13249. |
| pHC33259 | Procaspase-3(D28A) | Feeney et al. (2005) JBC 280, 39772. |
| pHC33260 | Procaspase-3(D9A,D28A) | Feeney et al. (2005) JBC 280, 39772. |
| pHC33263 | Procaspase-3(D9A,D175A) | Feeney et al. (2005) JBC 280, 39772. |
| pHC33264 | Procaspase-3(C163S,W206Y) |
No Reference |
| pHC33265 | Procaspase-3(C163S,W214V) | No Reference |
| pHC312 | Caspase-3 small subunit | Pop et al. (2001) Biochemistry 40, 14224. |
| pHC317 | Caspase-3 large subunit | Pop et al. (2001) Biochemistry 40, 14224. |
| pHC31701 | Caspase-3 large subunit, C163S | Pop et al. (2001) Biochemistry 40, 14224. |
| pHC319 | Caspase-3 large subunit plus pro-peptide | Pop et al. (2001) Biochemistry 40, 14224. |
| pHC31901 | Caspase-3 large subunit plus pro-peptide, C163S | Pop et al. (2001) Biochemistry 40, 14224. |
| pET28a-p12 | Caspase-3 small subunit with Kan resistance | No Reference |