A. Clay Clark

Raleigh, North Carolina, USA

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Mailing Address:

   Dr. A. Clay Clark
   Associate Professor
   128 Polk Hall, CB 7622
   Department of Molecular
       & Structural Biochemistry
   NC State University
   Raleigh, NC 27695-7622

   Send Email: Clay Clark

  Lab: Rooms 339 & 341 Polk Hall
  Office: Room 339A Polk Hall

  919-515-5805 (office)
  919-515-5806 (lab)
  919-515-2047 (fax)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CARD Expression Vectors

 

Click on the link for the sequence of each plasmid

Plasmid
Comments
Reference
pCARD-RICK CARD (caspase recruitment domain) of RICK
parent plasmid: pET21a
 Chen & Clark (2003) Biochemistry 42, 6310.
pCARD-procasp1 CARD of caspase-1
parent plasmid: pET21a 		Chen & Clark (2004) Protein Science 13, 2196.
pCARD-RICKP47A P47A mutant of RICK CARD
parent plasmid: pET21a 		Chen & Clark (2006) Protein Science 15, 395.
pCARD-RICKP85A P85A mutant of RICK CARD
parent plasmid: pET21a 		Chen & Clark (2006) Protein Science 15, 395.
pCARD-RICKP87A P87A mutant of RICK CARD
parent plasmid: pET21a 		Chen & Clark (2006) Protein Science 15, 395.
pCARD-RICKP47AP85A P47A,P85A double mutant of RICK CARD
parent plasmid: pET21a 		Chen & Clark (2006) Protein Science 15, 395.
pCARD-RICKP47AP87A P47A,P87A double mutant of RICK CARD
parent plasmid: pET21a 		Chen & Clark (2006) Protein Science 15, 395.
pCARD-RICKP85AP87A P85A,P87A double mutant of RICK CARD
parent plasmid: pET21a 		Chen & Clark (2006) Protein Science 15, 395.
pCARD-RICKP47AP85AP87A P47A,P85A,P87A triple mutant of RICK CARD, called P3A
parent plasmid: pET21a
 Chen & Clark (2006) Protein Science 15, 395.
TEV ProCARD Procaspase-1 CARD with TEV protease cleavage site
parent plasmid: pET21a 		No Reference
pGEX-4T2Pro Procaspase-1 CARD
parent plasmid: pGEX-4T2		 No Reference
pGEX-6P2Pro Procaspase-1 CARD
parent plasmid: pGEX-6P2		 No Reference
pGEX-4T2RICK RICK CARD
parent plasmid: pGEX-4T2		 No Reference
pGEX-6P2RICK RICK CARD
parent plasmid: pGEX-6P2		 No Reference
pApaf-1CARD-2T Apaf-1 CARD
parent plasmid: pGEX-2T		 Feeney et al. (2006) Protein Expression and Purification 47, 311.
pApaf-1CARD+GS Apaf-1 CARD with caspase-3 cleavage site (DELD). Leaves GS sequence at N-terminus.
parent plasmid: pGEX-2T 		Feeney et al. (2006) Protein Expression and Purification 47, 311.
pApaf-1CARD-GS Apaf-1 CARD with caspase-3 cleavage site (DELD). No GS sequence at N-terminus.
parent plasmid: pGEX-2T 		Feeney et al. (2006) Protein Expression and Purification 47, 311.