BRASSINOSTEROID INSENSTITIVE 1 (BRI1) At4G39400
MKTFSSFFLSVTTLFFFSFFSLSFQASPSQSLYREIHQLISFKDVLPDKNLLPDWSSNKN
PCTFDGVTCRDDKVTSIDLSSKPLNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSGFKC
SASLTSLDLSRNSLSGPVTTLTSLGSCSGLKFLNVSSNTLDFPGKVSGGLKLNSLEVLDL
SANSISGANVVGWVLSDGCGELKHLAISGNKISGDVDVSRCVNLEFLDVSSNNFSTGIPF
LGDCSALQHLDISGNKLSGDFSRAISTCTELKLLNISSNQFVGPIPPLPLKSLQYLSLAE
NKFTGEIPDFLSGACDTLTGLDLSGNHFYGAVPPFFGSCSLLESLALSSNNFSGELPMDT
LLKMRGLKVLDLSFNEFSGELPESLTNLSASLLTLDLSSNNFSGPILPNLCQNPKNTLQE
LYLQNNGFTGKIPPTLSNCSELVSLHLSFNYLSGTIPSSLGSLSKLRDLKLWLNMLEGEI
PQELMYVKTLETLILDFNDLTGEIPSGLSNCTNLNWISLSNNRLTGEIPKWIGRLENLAI
LKLSNNSFSGNIPAELGDCRSLIWLDLNTNLFNGTIPAAMFKQSGKIAANFIAGKRYVYI
KNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSRVYGGHTSPTFDNNGSMMFLD
MSYNMLSGYIPKEIGSMPYLFILNLGHNDISGSIPDEVGDLRGLNILDLSSNKLDGRIPQ
AMSALTMLTEIDLSNNNLSGPIPEMGQFETFPPAKFLNNPGLCGYPLPRCDPSNADGYAH
HQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRKRRRKKEAELEMYAEGHGNSGD
RTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQATNGFHNDSLIGSGGFGDVYKA
ILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEFMK
YGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHHNCSPHIIHRDMKSSNVLLDEN
LEARVSDFGMARLMSAMDTHLSVSTLAGTPGYVPPEYYQSFRCSTKGDVYSYGVVLLELL
TGKRPTDSPDFGDNNLVGWVKQHAKLRISDVFDPELMKEDPALEIELLQHLKVAVACLDD
RAWRRPTMVQVMAMFKEIQAGSGIDSQSTIRSIEDGGFSTIEMVDMSIKEVPEGKL
Peptides identified are underlined
Phosphorylated peptides identified by ion-trap are in red
Phosphorylated peptides identified by QTOF are in blue
Phosphorylated peptides identified by both instruments are in green
Total protein (sequence) coverage: 31.6%
Cytoplasmic domain (sequence) coverage: 65.7%
Ion trap data (NC State Univ)1
|
Phosphorylated peptides identified2 |
Calculated mass | Measured mass | Charge state | Sequest Xcorr |
y and b ions identified/predicted |
| EAELEMYAEGHGNS*GDR | 1960.9 | 1960.2 | 2 | 2.16 | 14/32 |
| EAELEMYAEGHGNS*GDR | 1960.9 | 1960.9 | 2 | 1.75 | 14/32 |
| AGVKLNWST#R | 1211.3 | 1211.1 | 2 | 1.79 | 7/18 |
| EIQAGSGIDSQS*TIR | 1641.6 | 1640.9 | 2 | 2.02 | 13/28 |
QTOF data (Michigan St. Univ)1
|
Phosphorylated peptides identified2 |
Calculated mass | Measured mass | Charge state | Mascot score |
y and b ions identified/predicted |
| EAELEMYAEGHGNS*GDR | 1960.9 | 1960.7 | 2 | 69 | 16/32 |
| EALS*INLAAFEKPLR | 1751.9 | 1752.8 | 2 | 89 | 16/28 |
| LT#FADLIQAT#NGFHNDSLIGSGGFGDVYK | 3218.3 | 3219.5 | 3 | 52 | 15/56 |
1 Mass spectral data identifying phosphorylation sites can be viewed by clicking on the peptide sequence
2 Phosphorylated residue is followed by either * (phosphoserine) or # (phosphothreonine)
These sites of phosphorylation were described in the following article:
BRASSINOSTEROID INSENSITIVE 1- ASSOCIATED RECEPTOR KINASE 1 (BAK1) At4G33430
MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDL
GNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNN
SLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITG
AIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGF
GKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCL
RERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTT
AVRGTIGHIAPEYLSTGKSSEKTDIFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVD
LQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIG
DSTSQIENEYPSGPR
Peptides identified are underlined
Phosphorylated peptides identified by ion-trap are in red
Total protein (sequence) coverage: 33.01%
Cytoplasmic domain (sequence) coverage: 50.68%
Ion trap data (NC State Univ)1
|
Phosphorylated peptides identified2,3 |
Calculated mass | Measured mass | Charge state | Sequest Xcorr |
y and b ions identified/predicted |
| ELQVASDNFS*NKNILGR | 1986.1 | 1984.9 | 2 | 3.43 | 21/48 |
| LADGT#LVAVK | 1067.2 | 1066.2 | 1 | 2.34 | 16/27 |
| LM@DYKDT#HVTTAVR | 1746.9 | 1747.3 | 3 | 3.57 | 32/78 |
| LM@DYKDTHVT#TAVR | 1746.9 | 1746.2 | 3 | 3.29 | 29/78 |
| LM@DYKDTHVT#TAVR | 1746.9 | 1745.7 | 2 | 3.06 | 22/39 |
| LMDYKDT#HVT#TAVR | 1810.8 | 1810.1 | 2 | 3.44 | 26/52 |
| GT#IGHIAPEYLSTGK | 1624.7 | 1625.4 | 3 | 3.30 | 33/48 |
1 Mass spectral data identifying phosphorylation sites can be viewed by clicking on the peptide sequence
2 Phosphorylated residue is followed by either * (phosphoserine) or # (phosphothreonine)
3 Oxidized methionine residue is followed by @