NC State Biochemistry receives state and federal revenues, but these sources represent a fraction of the support required to maintain successful programs and facilities. Your donations will make the difference for our faculty and students to meet the global challenges of 21st Century:

- Enhancing the production, quality, accessibility and proftability of food, plant, animal and bioenergy products for North   Carolina, the nation and the world
- Ensuring environmental stewardship and sustainability of air, land, soil and water resources
- Creating a food supply that is safe, secure, healthy, affordable and of high quality
- Improving human health and well-being for individuals, families and communities and
- Preparing students and stakeholders for leadership and success in the global workforce.

NC State Biochemistry



James A. Knopp
Alumni Association Distinguished Undergraduate Professor and Undergraduate Coordinator of Biochemistry

PhD, University of Illinois at Urbana
Postdoctoral, Oak Ridge National Laboratory

Office: 128 Polk Hall
   Office: 919.515.5828
Email: James Knopp

James Knopp

Research Areas: Particulate and microbial transfer | plant-pathogen interactions | digital fluorescence image analysis in vivo oxygen measurements | protein conformational analysis

Fabrics and flooring surfaces such as carpets and tiles are a potential source of many environmental toxins for humans. A small baby walking across a floor on hands and knees would be expected to pickup a significant amount of such toxins. I have been collaborating with textile chemists to develop reproducible and automated measurements of the transfer of various particulates and microorganisms from textile surfaces to human skin. Using these data, I have been developing a kinetic mathematical model for such transfer. Considerable effort and money is expended to remove these toxic materials through a variety of cleaning processes.

Previous research projects have included the use of fluorescence spectroscopic techniques to measure protein conformational change. A novel fluorescent probe based on pyrene butyric acid was developed and characterized. This probe also had the property of a long enough fluorescence lifetime to be sensitive to quenching by molecular oxygen. This latter property was exploited through the use of pyrene butyric acid as an in vivo and non-invasive probe of intra-cellular and intra-organ oxygen concentrations. This process involved the development of digital video image analysis of microscopic fluorescence images to calculate the intra-cellular oxygen concentration maps.

Other projects have involved the investigation of potassium transport during the hypersensitive reaction of tobacco cells to bacterial infection, of the effect of cell status during fungal infection of corn leaves, and of the possible roll of nitric oxide in the nematode infection of soybeans.