NC State Biochemistry receives state and federal revenues, but these sources represent a fraction of the support required to maintain successful programs and facilities. Your donations will make the difference for our faculty and students to meet the global challenges of 21st Century:

- Enhancing the production, quality, accessibility and proftability of food, plant, animal and bioenergy products for North   Carolina, the nation and the world
- Ensuring environmental stewardship and sustainability of air, land, soil and water resources
- Creating a food supply that is safe, secure, healthy, affordable and of high quality
- Improving human health and well-being for individuals, families and communities and
- Preparing students and stakeholders for leadership and success in the global workforce.


NC State Biochemistry

 

 

E. Stuart Maxwell
Professor of Biochemistry

Education:
PhD, University of Massachusetts, Amherst
Postdoctoral, Insitute Jacque Monod, Paris; University of Chicago

Contact:
Office: 332 Polk Hall
Phone:
   Office: 919.515.5803
Email: E. Stuart Maxwell

E. Stuart Maxwell

Website: Visit our Lab Home Page

Research Areas: Synthesis, structure and function of the small nucleolar RNAs

The small nucleolar RNAs (snoRNAs) play crucial roles in ribosome biosynthesis. Using complementary sequences, the snoRNAs base pair with rRNA to guide the folding, site-specific nucleotide modification, and cleavage of precursor ribosomal RNA. Based upon conserved snoRNA sequence elements, this large population of guide RNAs has been divided into two major families designated the box C/D and the box H/ACA snoRNAs. The primary function of the box C/D snoRNAs is to guide 2'-O-methylation of targeted rRNA nucleotides while the box H/ACA snoRNAs direct the conversion of specific uridine residues to pseudouridine.

Our laboratory is investigating the biogenesis, structure, and function of the box C/D RNP nucleotide modification complexes using U14 as a model system for the eukaryotic snoRNPs. Early work from our laboratory reported the first intronic snoRNA and we subsequently demonstrated a novel pre-mRNA intron processing pathway for snoRNA biosynthesis. Our work then defined the terminal box C/D core motif, a folded RNA element essential for snoRNA processing, snoRNP assembly, and snoRNP transport to the nucleolus.


Most Recent Publications:

2015
Assessing intermolecular RNA:RNA interactions within a ribonucleoprotein complex using heavy metal cleavage mapping.
Methods in molecular biology (Clifton, N.J.)
 1240: 125-34 
Gagnon KT | Maxwell ES |

2012 Jun 01
Structurally conserved Nop56/58 N-terminal domain facilitates archaeal box C/D ribonucleoprotein-guided methyltransferase activity.
The Journal of biological chemistry
 287: 19418-28 
Gagnon KT | Biswas S | Zhang X | Brown BA | Wollenzien P | Mattos C | Maxwell ES |

2011 Apr 12
Comparative analysis of the 15.5kD box C/D snoRNP core protein in the primitive eukaryote Giardia lamblia reveals unique structural and functional features.
Biochemistry
 50: 2907-18 
Biswas S | Buhrman G | Gagnon K | Mattos C | Brown BA | Maxwell ES |

2011
Electrophoretic mobility shift assay for characterizing RNA-protein interaction.
Methods in molecular biology (Clifton, N.J.)
 703: 275-91 
Gagnon KT | Maxwell ES |

2011 Jan
The spatial-functional coupling of box C/D and C'/D' RNPs is an evolutionarily conserved feature of the eukaryotic box C/D snoRNP nucleotide modification complex.
Molecular and cellular biology
 31: 365-74 
Qu G | van Nues RW | Watkins NJ | Maxwell ES |